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Figure 4 | Virology Journal

Figure 4

From: Receptor chimeras demonstrate that the C-terminal domain of the human cytomegalovirus US27 gene product is necessary and sufficient for intracellular receptor localization

Figure 4

Cell surface staining of US27. A) HEK293 cells were transiently transfected with the indicated p3XFLAG constructs, harvested via gently scraping, staining with anti-FLAG antibodies, then examined via flow cytometry using a BD FACSCalibur. Data was analyzed using CellQuestPro. B) Transiently transfected cells grown on glass coverslips were fixed and stained in the absence of any detergents or permeabilization steps. After treatment with FITC-conjugated anti-mouse secondary antibodies, the coverslips were mounted with Prolong Gold containing DAPI. Scale bars = 10 μm.

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Virology Journal

ISSN: 1743-422X