Figure 4From: Receptor chimeras demonstrate that the C-terminal domain of the human cytomegalovirus US27 gene product is necessary and sufficient for intracellular receptor localizationCell surface staining of US27. A) HEK293 cells were transiently transfected with the indicated p3XFLAG constructs, harvested via gently scraping, staining with anti-FLAG antibodies, then examined via flow cytometry using a BD FACSCalibur. Data was analyzed using CellQuestPro. B) Transiently transfected cells grown on glass coverslips were fixed and stained in the absence of any detergents or permeabilization steps. After treatment with FITC-conjugated anti-mouse secondary antibodies, the coverslips were mounted with Prolong Gold containing DAPI. Scale bars = 10 μm.Back to article page