Table 1 Primer sequences used in this study
Primers | Regions | Nucleotide sequences (5'→3') | Nucleotide position | Amplicons (bp) | References |
|---|---|---|---|---|---|
CA24-1F | 5'-UTR | GAAATTAAAACAGCTCTGGGGTTGTTCC | 1~820 | 820 | This study |
CA24-1R | VP4 | TGGATCCGCCAGTTGCCACATTAG | Â | Â | Â |
CA24-2F | 5'-UTR | GGCAACACTATTACAATGGGTGCC | 736~1620 | 885 | This study |
CA24-2R | VP2 | GGCCATACAGTCTATTGCTAGGGAG | Â | Â | Â |
CA24-3F | vp2 | CGAATAATTCGGCAACTCTTGTGCTG | 1558~2523 | 965 | This study |
CA24-3R | vp1 | AGTAATAACGGTGTCAATGGTCTCC | Â | Â | Â |
CVA24v-S | VP3 | GTGAGTGCTTGCCCAGATTT | 2407~3438 | 1032 | Ding et al. 2009 |
CVA24v-A | 2a | ATACACCGCCATGTTCTGGT | Â | Â | Â |
CA24-5F | vp1 | TAAGGTGTTGGTGTCCTAGACCG | 3292-4197 | 905 | This study |
CA24-5R | 2c | CCCTTTGGCAGCATTACAGGC | Â | Â | Â |
CA24-6F | 2b | GCGACTGTTCACCCTGGC | 4072-4853 | 781 | This study |
CA24-6R | 2c | TCGCTGTGTGAGACTGTTGGAG | Â | Â | Â |
CA24-7F | 2c | GCAACGACATGAAGCTGTTCTGTC | 4696-5322 | 626 | This study |
CA24-7R | 3a | CAAAACAGTCATGGCCCTGTTCAG | Â | Â | Â |
3C-1 | 3a | AAAGGGATGGATCGTCAAGC | 5250~6181 | 932 | Wu et al.2008 |
3C-2 | 3d | TAGCCTCTTCAAAGTCTGTC | Â | Â | Â |
CA24-9F | 3d | GAGCCCAGTGTCTTCCATTGTG | 6084-6748 | 664 | This study |
CA24-9R | 3d | CGAACCATGCAGGACTGAGTGA | Â | Â | Â |
CA24-10F | 3d | CCAGGCGTAGTGACAGGATCAG | 6612-7461 | 849 | This study |
CA24-10R | 3'-UTR | CCGAATTAAAGAAAAATTTACCCCTACAAC | Â | Â | Â |