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Table 4 Shelf life of ELISA assay at 4°C and -20°C

From: Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito

 

4°C

-20°C

 

Positive Control

(mean+SD)

Negative Control

(mean+SD)

Positive Control

(mean+SD)

Negative Control

(mean+SD)

One Month

0.962 ± 0.037

0.168 ± 0.009

0.898 ± 0.058

0.155 ± 0.061

Two Months

0.934 ± 0.041

0.155 ± 0.014

0.845 ± 0.025

0.147 ± 0.039

Three Months

0.878 ± 0.014

0.145 ± 0.023

0.797 ± 0.006

0.115 ± 0.011

Four Months

0.863 ± 0.023

0.127 ± 0.008

0.752 ± 0.009

0.104 ± 0.008

Five Months

0.856 ± 0.005

0.139 ± 0.012

0.793 ± 0.052

0.113 ± 0.043

Six Months

0.832 ± 0.034

0.143 ± 0.051

0.721 ± 0.048

0.133 ± 0.029

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Virology Journal

ISSN: 1743-422X