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Table 2 Repeatability of ELISA assay for 5 different samples

From: Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito

 

Detection No. ELISA OD630

    

Specimen No.

Plate 1

Plate 2

AVG

SD

CV

p-Value

 

1

2

3

4

5

6

    

1

0.46

0.499

0.535

0.475

0.442

0.49

0.484

0.0325

6.70%

0.1626

2

0.231

0.204

0.207

0.211

0.213

0.192

0.21

0.0128

6.10%

0.2346

3

0.126

0.126

0.125

0.148

0.12

0.127

0.129

0.0098

7.60%

0.2577

  1. AVG Average of 6 detection OD630 values for each sample
  2. SD Standard deviation of 6 detection OD630 values for each sample
  3. CV Coefficient of variation of 6 detection OD630 values for each sample
  4. p-Value The significance difference analysis between plate 1 and plate 2 as inter-repeat assay