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Table 1 Sequences of PCR primers used to construct luciferase reporter vectors containing different fragments of the human IVL promoter

From: Effects of human papillomavirus (HPV) type 16 oncoproteins on the expression of involucrin in human keratinocytes

Plasmid construct

Primer sequence (5' to 3')

Restriction site

pGL3-IVL-2418

GCGCCTCGAG CAGTGAAAGAACCTCTCCCA

Xho I

 

GCGCAAGCTT CCATCCGACACTTACCAGAC

Hind III

pGL3-IVL-1809

GCGCCTCGAG ATGATCCAGGAACATGACAA

Xho I

 

GCGCAAGCTT CCATCCGACACTTACCAGAC

Hind III

pGL3-IVL-744

GCGCCTCGAG TTCTTATGAGCATGGCATTC

Xho I

 

GCGCAAGCTT CCATCCGACACTTACCAGAC

Hind III

pGL3-IVL-272

GCGCCTCGAG AGTTGAGCTACCAGAATCCT

Xho I

 

GCGCAAGCTT CCATCCGACACTTACCAGAC

Hind III

  1. PCR products amplified using the indicated primers were digested by Xho I and Hind III and cloned into the luciferase reporter vector pGL3
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Virology Journal

ISSN: 1743-422X