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Figure 1 | Virology Journal

Figure 1

From: Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions

Figure 1

Determining the optimal temperature and time for LAMP. (A) Determination of the optimal temperature. Lane M, DL-2000 DNA marker; Lanes 1–9: Reaction for 60 min at 58, 59, 60, 61, 62, 63 and 64°C; negative control; and reaction at 65°C. (B) Determination of the optimal time. Lane M, DL-2000 DNA marker; Lanes 1–5 and 7: Reaction for 10, 20, 30, 40, 50 and 60 min (65°C), respectively; Lane 6: –, negative control. All LAMP products were electrophoresed on 2% agarose gels and stained with ethidium bromide.

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Virology Journal

ISSN: 1743-422X