Table 3 Primers used for screening and cloning of TTV DNA
S.No | Primer name | Purpose | Sequence | Position | Expected product size |
|---|---|---|---|---|---|
1. | T1F | Screening | 5′--GTA AGT GCA CTT CCG AAT GGC TGA G-3′ | 91-115 | 133 bp |
| Â | (NG133) | Â | Â | Â | Â |
2. | T1R | Screening | 5′--AGC CCG AAT TGC CCC TTG AC-3′ | 204-223 |  |
| Â | (NG132) | Â | Â | Â | Â |
3. | TTF2 | Cloning | 5′- TAT GTC GTC CAC TTC CTG GG-3′ | 50-69 | 3652 bp |
4. | TTR2 | Cloning | 5′- GAG GAA GGA AGT CGG CCA TTT TG-3′ | 3684-3707 |  |