Neutralization of MLV pseudotyped virus bearing envelopes from different viruses. β-galactosidase-expressing MLV pseudotypes bearing the GP1,2 of EBOV, SUDV, TAFV, or BDBV or the G protein of VSV were pre-incubated with the pooled sera of each group (VLP (n = 5), DNA (n = 6), DNA/VLP (n = 7)), which were collected at week 40, at the final concentration of 1:25 dilution. The control group represents a pool of sera collected from one mouse in each of the negative control groups: CpG, CpG/PBS, and PBS. Vero cells were exposed to virus/antibody mixtures and the virus titer was quantified by counting the number of blue forming units (BFU) under microscope. Two or three replicates were used in each experiment. For each pseudotype, the titer observed in the presence of sera pooled from negative control mice was normalized to 100%, and all other titers for that particular pseudotype are reported as relative to that value. Representative data from two or more experiments are shown.