Skip to main content
Figure 3 | Virology Journal

Figure 3

From: Induction of ebolavirus cross-species immunity using retrovirus-like particles bearing the Ebola virus glycoprotein lacking the mucin-like domain

Figure 3

Antibody response. Two weeks after the third immunization, sera were collected and pooled for each group. The anti-GP1,2 antibody titers for the DNA group (A) and DNA/VLP group (B) were determined using a sandwich ELISA. GP1,2-Fc or the negative control Fc was captured to the ELISA plate using an anti-Fc antibody. Antibody titer was defined as the highest dilutions where the OD of the sample was higher than 0.15 and at least two times that of the control. The same assay was used to determine the dynamics of anti-GP1,2 antibodies for the DNA group (C, all diluted at 1:64) and DNA/VLP group (D, all diluted at 1:2,000). The syringe symbol indicates the time points when mice were injected with the indicated immunogen. (E) Sera from mice immunized with DNA/VLP or VLP were collected 2 weeks after the third immunization and analyzed by western blot against lysates from cells transiently expressing full-length GP1,2 (E, EBOV) or from cells transfected with no plasmid DNA (M, mock)

Back to article page