|
PCR reaction / Primer (Polarity)
|
Sequence (5′to 3′)
|
Positiona
|
PCR product
|
|---|
|
Primary
| | |
508 bp
|
|
HPIV2-cDNA (+)
|
AACAATCTGCTGCAGCATTT
|
7437-7456
|
|
HPIV2-rev (−)
|
ATGTCAGACAATGGGCAAAT
|
7925-7944
|
|
Nested
| | |
204 bp
|
|
HPIV2-nF(+)
|
CCATTTACCTAAGTGATGGAAT
|
7479-7500
|
|
HPIV2-nR(−)
|
GCCCTGTTGTATTTGGAAGAGA
|
7661-7682
|
|
Sequencing
| | | |
|
HPIV2-HN-F1(+)
|
CGAACCCTTAAGGTGTCGTAACGTC
|
6648-6672
|
886 bp
|
|
HPIV2-HN-R1 (−)
|
GGTATAGCAGTGACTGAACAGC
|
7512-7533
| |
|
HPIV2-HN-F2(+)
|
GACCCATTGGTGTTACACTCACAATG
|
7348-7373
|
840 bp
|
|
HPIV2-HN-R2(−)
|
GTTGCATTGCATGGCATGACTC
|
8166-8187
| |
|
HPIV2-HN-F3(+)
|
GGTACCGTCCTATCAAGTTCC
|
8137-8157
|
426 bp
|
|
HPIV2-HN-R3 (−)
|
GAATCTGGAGTCTTCATTCAATAG
|
8539-8562
| |
- a Nucleotide positions based on the complete genome sequence of HPIV-2 strain Greer; GenBank accession number AF533012.
