Table 1 Oligonucleotide primers used in the study
PCR reaction / Primer (Polarity) | Sequence (5′to 3′) | Positiona | PCR product |
|---|---|---|---|
Primary | Â | Â | 508 bp |
HPIV2-cDNA (+) | AACAATCTGCTGCAGCATTT | 7437-7456 | |
HPIV2-rev (−) | ATGTCAGACAATGGGCAAAT | 7925-7944 | |
Nested | Â | Â | 204 bp |
HPIV2-nF(+) | CCATTTACCTAAGTGATGGAAT | 7479-7500 | |
HPIV2-nR(−) | GCCCTGTTGTATTTGGAAGAGA | 7661-7682 | |
Sequencing | Â | Â | Â |
HPIV2-HN-F1(+) | CGAACCCTTAAGGTGTCGTAACGTC | 6648-6672 | 886 bp |
HPIV2-HN-R1 (−) | GGTATAGCAGTGACTGAACAGC | 7512-7533 |  |
HPIV2-HN-F2(+) | GACCCATTGGTGTTACACTCACAATG | 7348-7373 | 840 bp |
HPIV2-HN-R2(−) | GTTGCATTGCATGGCATGACTC | 8166-8187 |  |
HPIV2-HN-F3(+) | GGTACCGTCCTATCAAGTTCC | 8137-8157 | 426 bp |
HPIV2-HN-R3 (−) | GAATCTGGAGTCTTCATTCAATAG | 8539-8562 |  |