Table 1 Oligonucleotides used for plasmid construction
Plasmid | contains | Oligonucleotide | Sequence (5'-3')* |
|---|---|---|---|
pMA319 | UL51 | MOL269 | ATGTCCTGGGCTAAGCAGCG |
MOL270 | TTATTTACCCGGCGCCGACT | ||
pMA48 | UL56 (5’) | MOL60 | CCATGG AGATGAATTTGTTAC |
MOL64 | CTCCTCGAGAATATGCTTGAT | ||
pMA49 | UL56 (3’) | MOL59 | ACAGGTTAACGCAGACTACCA |
MOL65 | ATATTCTCGAGGAGATTCGAC | ||
pUL89 | UL89 cDNA | MOL53 | CTGCTGCGTGCCTAGCTGAC |
MOL54 | CATCATGTTGCGCGGAGACT | ||
pMA323 | FLAG-UL51 | UL51-FLAG-H3F | GACCTAAGCTT TCCTGGGCTAAGCAGCGGGTG |
UL51-FLAG-ER1R | TACGATGAATTC TTATTTACCCGGCGCCGACTC | ||
pMA326 | FLAG-UL51 | UL51-FLAG-BamHI | CGGGATCC ATGGACTACAAGGACGACGA |
UL51-FLAG-ER1R | TACGATGAATTC TTATTTACCCGGCGCCGACTC | ||
pMA325 | UL56 | UL56-V5-F | GAGATGAATTTGTTACAGAAACTA |
UL56-V5-R | TTAACGCAGACTACCAGGCAC | ||
pMA324 | MYC-UL89 | linkA | GATCCACCATGGAACAAAAACTCATCTCAGAAGAGGATCTGG |
linkB | AATTCCAGATCCTCTTCTGAGATGAGTTTTTGTTCCATGGTG | ||
UL89-NMyc-ER1F | CATGATGAATTC TTGCGCGGAGACTCGGCC | ||
UL89-NMyc-XB1R | TACATGTCTAGA CTAGCTGACCCTGAAACG |