Table 1 List of primers used
From: Mouse macrophage innate immune response to chikungunya virus infection
Gene | Primer 1 (5′→ 3′) | Primer 2 (5′→ 3′) | Amplicon (bp) |
|---|---|---|---|
GAPDH | GAACGGGAAGCTTGTCATCA | TGACCTTGCCCACAGCCTTG | 473 |
IFNα4 | AGCCTGTGTGATGCAGGAACC | CAGCAAGTTGGTTGAGGAAGAG | 171 |
ISG56 | CTCAGAGCAGGTCCAGTTCC | TCCATCTCAGCACACTCCAG | 300 |
TNFα | ACGGCATGGATCTCAAAGAC | CGGACTCCGCAAAGTCTAAG | 325 |
CHIKV_E1 | AAGCTYCGCGTCCTTTACCAAG | CCAAATTGTCCYGGTCTTCCT | 209 |
Probe_E1 | FAM – CCAATGTCYTCMGCCTGGACACCTTT - TAMRA | ||
CHIKV_E2 | ACKCTGACRGTGGGATTYAC | ACGTGCTGCAAGGTARCTCT | 139 |
CHIKV_Pos | ACGTGCTGCAAGGTARCTCT | ||
CHIKV_Neg | ACKCTGACRGTGGGATTYAC |