Inhibition of CVB3 and up-regulation of IFNs by MAVS introduction after CVB3 infection. After exposure to CVB3 for 1 h, pcDNA3.1 and pcDNA3.1-FLAG-MAVS were introduced into HeLa cells. In 0 h ~ 36 h, cells were harvested and total RNA was extracted. (A) CVB3 RNA, (B) IFN-β mRNA and (C) IFN-α mRNA were detected through RT-qPCR. * p < 0.05,** p < 0.01.