Inhibition of CVB3 by exogenous MAVS in pre-infected HeLa cells. HeLa cells were challenged by CVB3-mCherry (absorption for 1 h) and then transfected with p-EGFP and p-MAVS-EGFP. (A) MAVS-GFP expression (green), CVB3 infection (red) and CPE were observed at 24 h and 48 h. (B) The percentage of infected cells with red fluorescence was calculated. (C) MTT was performed to determine the cell viability of HeLa cells inoculated with CVB3 at MOI of 0, 0.001, 0.01, 0.1, 1 and then transfected with p-EGFP and p-MAVS-EGFP plasmids (48 h post-transfection). * p < 0.05,** p < 0.01.