Restriction of CVB3 caused CPE by exogenous MAVS in HeLa cells. At 24 h post-transfected with p-EGFP or p-MAVS-EGFP, HeLa cells were challenged by CVB3-mCherry. After 24 h and 48 h of CVB3-mCherry infection, (A) the expression of MAVS-GFP (green), CVB3-mCherry infection (red) and the CPE were observed. (B) The percentage of infected cells was calculated. (C) CVB3-mCherry was used to inoculate HeLa cells (MOI = 0, 0.001, 0.01, 0.1, 1) after transfection with p-EGFP or p-MAVS-EGFP. MTT test was used to determine the cell viability. (D &E) Cells over-expressing exogenous MAVS were exposed to CVB3-woodruff or CVB3-mCherry respectively, and the survival cells were stained by crystal violet. The percentage of survival cells was evaluated by observation. (F) HeLa cells were transfected with p-EGFP or p-MAVS-EGFP for 24 h and harvested for RNA extraction, and RT-qPCR was performed to verify the expression level of exogenous MAVS. * p < 0.05,** p < 0.01.