Time and dose effect of MAVS on cell growth and IFN-β production. HeLa cells were transfected with pcDNA 3.1 and pcDNA3.1-FLAG-MAVS. (A) At 24 h post-transfection, fusion protein of FLAG-MAVS was detected by western blotting. (B) Cell proliferation was evaluated through trypan blue dye exclusion. (C) At 24 h post-transfection, RT-qPCR was performed to evaluate the IFN-β transcriptional level at different dosages of MAVS construct transfection. (D) The concentration of IFN-β in culture supernatant was determined with ELISA at different time points post-transfection. * p < 0.05,** p < 0.01.