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Table 1 Titer assessment for HHV-6A GS strain batches expressed as TCID 50 determined by Q-PCR, ocular inspection or immunofluorescence assay (IFA), and as infectious units (Inf U) determined by IFA

From: Development and validation of a Q-PCR based TCID50 method for human herpesvirus 6

Batch Q-PCR Ocular inspection Inf U/ml IFA HHV-6A
(passage) TCID50/ml TCID50/ml   TCID50/ml DNA cop/ml
P17 1215 ±566 (x3)1 771 ±466 (x3) nd2 972 ±719 (x2) 7.6e8 ±1.4e8 (x2)
P19 806 ±679 (x5) 465 ±408 (x5) 2.5e4 ±1.6e4 (x3) 649 N/A3 (x1) 16.0e8 ±1.3e8 (x2)
P21 6 ±8 (x2) 14 ±6 (x2) nd nd 7.7e8 ±0.15e8 (x2)
P27 7 ±6 (x3) 30 ±15 (x3) 3.9e4 N/A (x1) 13 ±3 (3x) nd
P17 UV-inact. 0 N/A (x1) 0 N/A (x1) nd nd nd
P17 heat-inact. 20 N/A (x1) 4 N/A (x1) nd nd nd
  1. 1 Number of replications of titer assessments for each passage.
  2. 2 nd: Not done.
  3. 3 N/A: Not applicable.
  4. Ocular inspection was performed by two independent assessors. Data presented are means ± standard deviations.