Table 1 Titer assessment for HHV-6A GS strain batches expressed as TCID 50 determined by Q-PCR, ocular inspection or immunofluorescence assay (IFA), and as infectious units (Inf U) determined by IFA
From: Development and validation of a Q-PCR based TCID50 method for human herpesvirus 6
Batch | Q-PCR | Ocular inspection | Inf U/ml | IFA | HHV-6A |
|---|---|---|---|---|---|
(passage) | TCID50/ml | TCID50/ml | Â | TCID50/ml | DNA cop/ml |
P17 | 1215 ±566 (x3)1 | 771 ±466 (x3) | nd2 | 972 ±719 (x2) | 7.6e8 ±1.4e8 (x2) |
P19 | 806 ±679 (x5) | 465 ±408 (x5) | 2.5e4 ±1.6e4 (x3) | 649 N/A3 (x1) | 16.0e8 ±1.3e8 (x2) |
P21 | 6 ±8 (x2) | 14 ±6 (x2) | nd | nd | 7.7e8 ±0.15e8 (x2) |
P27 | 7 ±6 (x3) | 30 ±15 (x3) | 3.9e4 N/A (x1) | 13 ±3 (3x) | nd |
P17 UV-inact. | 0 N/A (x1) | 0 N/A (x1) | nd | nd | nd |
P17 heat-inact. | 20 N/A (x1) | 4 N/A (x1) | nd | nd | nd |