Figure 2From: Development and validation of a Q-PCR based TCID50 method for human herpesvirus 6Determination of cut-point for infection. The optimal cut-point for infection was found to be a ten times increase where the correlation to protein expression was seen in 93% of the wells by IFA with an antibody targeting the late viral protein gp116/54/64. No well contained viral protein where the viral DNA load had not increased ten times. Data shown are mean results (± SEM) from three TCID50 plates.Back to article page