in vitro transcription with recombinant L-P complex. (a) Time kinetics. Transcription reaction mixture containing 10μg of r(L-P) complex purified from Sf 21 cells were incubated at 30°C for the indicated time. The incorporation of 32P-UMP in RNA was monitored by TCA precipitation. A parallel reaction with N-RNA alone was carried out as control. Data represents the average of two independent experiments. (b) Enzyme concentration dependency of RNA synthesis.