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Figure 2 | Virology Journal

Figure 2

From: Establishment of an in vitro transcription system for Peste des petits ruminant virus

Figure 2

(a) in vitro transcription with RNP complex-Time kinetics of RNA synthesis. Transcription reaction mixture containing 20 μg of RNP complex purified from PPR infected Vero cells were incubated at 25, 30 and 37°C for different times. The incorporation of 32P-UMP into RNA was monitored by measuring TCA insoluble radioactivity. Transcription reaction in the absence of RNP complex was used as control. (b) in vitro transcription with RNP complex-dependency on protein concentration. Transcription reaction mixtures containing different amounts of RNP complex were incubated at 30°C for two hours.

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