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Figure 3 | Virology Journal

Figure 3

From: Permissiveness of human hepatoma cell lines for HCV infection

Figure 3

HCVpp and HCVcc infection in PLC, Hep3B and HepG2-CD81 cells exogenously over expressing HCV entry factors. Huh7, PLC, Hep3B and HepG2-CD81 cells transiently transfected with a vector control plasmid or expression plasmids expressing CD81, SR-BI, CLDN1 and/or OCLN were infected with (A-C) equal amounts of JFHpp, H77pp or VSVGpp or (D-F) HCVcc at an MOI of 2.0 FFU/cell. HCVpp entry (following background and mock-control signal subtraction) is expressed as relative light units (RLU) ± SD for triplicate samples determined 72 h p.i. Intracellular HCVcc RNA was quantified by RTqPCR, normalized to GAPDH and is displayed as mean HCV RNA copies/μg total cellular RNA ± SD for triplicate samples determined 72 h p.i.

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