Figure 7
From: Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells
Polar entry and release of PUUV from MDCKII cells. A) Polarized MDCKII monolayers were infected with PUUV and hantaviral nucleocapsid protein was detected by immunofluorescence. B) Entry of PUUV in polarized MDCKII cells was analyzed by the detection of nucleocapsid protein in cell lysates by Western blot. C) MDCKII cells were inoculated with PUUV via the apical or basolateral chamber. Cell-free supernatants of infected polarized MDCKII cells were analyzed for the presence of PUUV nucleocapsid protein by Western blot. D) MDCKII cells were inoculated with PUUV via the apical or basolateral chamber. Supernatants of the apical and basolateral chamber of MDCKII cells were collected and added to Vero E6 cells. Re-infection of Vero E6 cells was analyzed by immunofluorescence of hantaviral nucleocapsid protein.