Figure 4
From: Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells
Productive infection of MDCKII cells with HTNV. MDCKII cells were infected with HTNV and analyzed for the presence of nucleocapsid protein by immunofluorescence and Western blot. Equal loading in cellular lysates uninfected and HTNV-infected cells was controlled by detection of tubulin. Release of particles was monitored by detection of nucleocapsid protein in cell-free supernatant of infected MDCKII cells. Release of infectious particles was assessed by re-infection of Vero E6 and MDCKII cells with supernatants of infected MDCKII cells and detection of nucleocapsid protein of HTNV by immunofluorescence.