Figure 1
From: Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells
Release of hantaviruses HTNV and PUUV from the apical surface of Vero C1008 cells. A) Polarized Vero C1008 cells were infected with HTNV and PUUV via the apical surface and cell-free supernatants of apical and basolateral chambers were analyzed for the presence of hantavirus nucleocapsid protein by Western blot. B) Re-infection of Vero E6 cells with supernatants (SN) of polarized Vero C1008 cells infected with HTNV and PUUV. Cells were analyzed for the expression of nucleocapsid protein of HTNV and PUUV by immunofluorescence at day 4 and 8 post infection, respectively.