Figure 2
Successful ultrasound guided injection of left ventricle wall. A) Needle (white arrows) was inserted in between the ribs into the left ventricle wall (outlined in blue) of anesthetized mouse. The needle tip (green arrow) was positioned intramyocardially without puncturing the left ventricle lumen. B) A liquid cloud (red circle) becomes visible and is retained in the ventricle wall after successful injection.