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Figure 2 | Virology Journal

Figure 2

From: Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo

Figure 2

DNA binding activity of NF-κB in CSFV-infected PBMCs. Cells treated with Mock, LPS (10ng/mL), Thiverval or GXW-07 at an MOI of 1 were obtained from pigs at 1, 4 and 7d, respectively. Nuclear extracts were prepared and subjected to EMSA with a Digoxin labeled NF-κB probe. Anti-p65 rabbit polyclonal antibody was used in supershift assay. A 100-fold molar excess of unlabeled NF-κB probe (unlabeled competitor) was added to analyze the specific bands. The experiment was repeated three times and the figure shows a representative experiment.

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