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Table 1 Oligonucleotide primers designed for SPV amplification by conventional gel-based PCR and real-time PCR

From: Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus

Primer

Sequence (5′-3′)

Length

  

Primer (bp)

Product (bp)

Nf

GGGGGATATGATTTTACCTTA

21

235a

Nr

ATATACCGTTTTTCATTTCGTTAG

24

 

900f

TTATATGTTATACCAATCGTTGGTC

25

900b

900r

TAACATACCTGCTAAAAACCAT

22

 
  1. a Length of product amplified by real-time PCR primer pair.
  2. b Length of product amplified by conventional PCR primer pair.
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Virology Journal

ISSN: 1743-422X