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Table 5 Specification characteristics of ELISA and rapid kits used for evaluation

From: Performance and diagnostic usefulness of commercially available enzyme linked immunosorbent assay and rapid kits for detection of HIV, HBV and HCV in India

Kit Type

Kit Name

Company Name

Kit Specifications

Detectable Ag/Ab

HIV ELISA

Microlisa-HIV

J. Mitra & Co. Pvt Ltd.

Indirect ELISA, recombinant antigens for HIV-1: gp41, C-terminus of gp120; for HIV-2: gp36 used in MWP.

IgG

Enzaids HIV 1 + 2 ELISA

SPAN Diagnostics Ltd.

Indirect ELISA, a combination of recombinant and synthetic peptides, derived from immuno-dominant regions of HIV-1 (gp120, gp41) and HIV-2 (gp36) used in MWP.

IgG

ERBA LISA HIV 1 + 2

Transasia Biomedicals Ltd.

Indirect ELISA, synthetic peptide of HIV 1 & 2 coated onto MWP.

IgG

HBsAg ELISA

Hepalisa

J. Mitra & Co. Pvt Ltd.

Direct sandwich, microwell plates coated with immobilized HBsAg antibodies

HBsAg

Microscreen HBsAg ELISA

SPAN Diagnostics Ltd.

Direct non-competitive ELISA, microwell plates coated with immobilized HBsAg antibodies

HBsAg

ERBA LISA HEPATITIS B

Transasia Biomedicals Ltd.

Sandwich method, microwell plates coated with immobilized HBsAg antibodies

HBsAg

HCV ELISA

Microlisa HCV

J. Mitra & Co. Pvt Ltd.

Indirect ELISA, 3rd generation tests and utilizes recombinant antigens from CORE, E1, E2, NS3, NS4 and NS5 genomic regions

IgG

Innova HCV ELISA

SPAN Diagnostics Ltd.

Indirect ELISA, 3rd generation test with highest sensitivity (100%) ensured by use of recombinant antigens from core, E1, E2, NS3, NS4 & NS5 genomic regions.

IgG

ERBA LISA HEPATITIS C

Transasia Biomedicals Ltd.

Indirect ELISA, 3rd generation tests, utilizes a combination of antigens from CORE, NS3, NS4 and NS5 genomic regions

IgG

HIV RAPID

HIV Comb

J. Mitra & Co. Pvt Ltd.

Dot Immunoassay, utilizes recombinant antigens for HIV-1: gp41, C-terminus of gp120; for HIV-2: gp36.

IgG

Combaids-RS Advantage

SPAN Diagnostics Ltd.

Dot Immunoassay, a combination of recombinant and synthetic peptides from immuno-dominant regions of HIV-1 (gp120, gp41) & HIV-2 (gp36).

IgG

SD BIOLINE HIV ½ 3.0

Standard Diagnostics, INC

Rapid immunochromatographic tests, membrane strip precoated with recombinant HIV-1 capture antigen (gp41, p24) on test band 1 region and with recombinant HIV-2 capture antigen (gp36) on test band 2.

IgG

HBsAg RAPID

HEPACARD

J. Mitra & Co. Pvt Ltd.

One step, rapid, visual and qualitative immunochromatographic assay based on the antigen capture or “sandwich” principle, utilizes monoclonal antibodies for HBsAg.

HBsAg

Crystal HBsAg

SPAN Diagnostics Ltd.

One step, rapid visual immunochromatographic tests for qualitative detection of Hepatitis B surface antigen (HBsAg), utilizes highly specific monoclonal antibodies ensure no cross reactivity with HAV and HCV

HBsAg

SD BIOLINE HBsAg

Standard Diagnostics, INC

One step, rapid, visual and qualitative immunochromatographic assay, membrane strip precoated with mouse monoclonal anti-HBs capture antibody on test band.

HBsAg

HCV RAPID

HCV-Comb

J. Mitra & Co. Pvt Ltd.

4th generation Dot Immunoassay, utilizes a unique combination of modified HCV antigens from the putative core, NS3, NS4 & NS5 genomic regions

IgG

Signal HCV

SPAN Diagnostics Ltd.

3rd generation immuno-dot test comprising of recombinant antigens from core, E1,E2,NS3,NS4 & NS5 genomic regions of HCV

IgG

SD BIOLINE HCV

Standard Diagnostics, INC

One step, rapid, visual and qualitative immunochromatographic assay, membrane strip precoated with recombinant HCV capture antigen (CORE, NS3, NS4 and NS5) on test band.

IgG