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Table 2 Serum and mucosal antibody responses in mice induced by DNA prime-intranasal proteinboost strategy based on NP

From: Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein

Group

Immunization

Ab responses (ELISA, 2n)a

Serum IgG subclasses (ELISA, 2n)a

IgG2a/ IgG1 Ratio

DNA prime

rNP boost

Serum IgG

Nasal wash IgA

Lung wash IgA

IgG2a

IgG1

D1

once

-

11.40±2.17

-

-

11.83±2.71

10.25±0.96

2.99

D2

twice

-

17.00±0.70

-

-

18.44±1.23

16.10±0.79

5.06

P1

-

once

18.20±0.45

0.89±0.19

6.50±0.53

15.50±1.37

17.89±1.01

0.19

D1P1

once

once

21.60±0.89b, c

2.67±0.46c

8.67±0.87c

21.55±1.50

19.25±0.82

4.92

D2P1

twice

once

24.80±0.83c, d, e

4.56±0.38c, e

11.30±1.12c, e

24.11±1.00

20.80±0.92

9.92

Control

-

-

-

-

-

-

-

-

  1. Mice were immunized as described in section of methods. 3 weeks after the last immunization, serum, nasal wash, and lung wash specimens of five mice in each group were prepared and examined by ELISA for NP-specific IgG, IgA, IgA Abs respectively.
  2. a Results are expressed as mean ± SD of five tested mice in each group.
  3. b Displays significant difference compared with mouse in D1group (P<0.05).
  4. c Displays significant difference compared with mouse in P1 group (P<0.05).
  5. d Displays significant difference compared with mouse in D2 group (P<0.05).
  6. e Displays significant differences compared with mouse in D1P1 group (P<0.05).