Table 2 Primers and vectors used to generate WNV recombinant proteins
Name | Position AF260967 | Primer forward | Primer reverse | Vector |
|---|---|---|---|---|
WARF4 | 7310-7757 | 5′-atggcggatcc aggcaatgcgctcagcccagcg-3′ BamHI | 5′- tctttgaagctt ctagtgaactcttcttttgtc-3′ HindIII | pRSET B |
ENV | 1852-2198 | 5′- ggaaaggatcc agttgaagggaacaacc -3′ BamHI | 5′-ctcctgaattc gtggttgtaaaggctttgcc-3′ EcoRI | pRSET C |
preM/M | 464-968 | 5′-cagcgggatcc cagttaccctctctaacttcc-3′ BamHI | 5′-caagggaattc aagctgtaagctggggccacc-3′ EcoRI | pRSET C |
Partial NS5 | 9015-9645 | 5′-gaggcggatcc gcggggggaatgtcacac-3′ BamHI | 5′-ttccgaattc tcaaacagccaggtcctg-3′EcoRI | pRSET B |
Partial NS4B | 7276-7680 | 5′-tcgatggatcc gacactatgcctacatggttcc-3′BamHI | 5′-ggatcaagctt ttatctttttagtcctggtttttc-3′HindIII | pRSET C |