Figure 2
From: Biological characteristics of the rtA181T/sW172* mutant strain of Hepatitis B virus in animal model
Time-dependent viral transcription after hydrodynamic transfection in vivo. A: HBV RNA of mice liver were detected by Northern blotting, and GAPDH transcript was used as an internal control for RNA loading per lane; B: The 3.5-kb HBV mRNA was quantitatively calculated automatically by the Quantity-One software (Bio-Rad); C or D: The total levels of HBV S-mRNA or C-mRNA in mouse liver were detected by qPCR, and the mean value of Wt of day 1 was defined as 1.