Table 4 Examples of TR-His-TMV-CP 19 crystals in Figure 5
From: The development and application of new crystallization method for tobacco mosaic virus coat protein
Examples | Protein Concentration | Crystal Conditions | Crystal appearance | Resolutions |
|---|---|---|---|---|
A | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.1 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 6.8 | The typical dodecahedral crystals were rapid growth (approximately 24 hr) at 295 K | >7.0 Å |
B | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.1 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.5. | The irregular octahedral crystals were rapid growth (approximately 24 hr) at 295 K | -- |
C | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.1 mol/L ammonium sulfate and 0.2 mol/L Tris/HCl, pH 7.0. | The irregular octahedral crystals were rapid growth (approximately 24 hr) at 295 K | -- |
D | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.3 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The typical octahedral crystals were rapid growth (approximately 24 hr) at 295 K | -- |
E | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 6.8. | The octahedral crystals were rapid growth (approximately 24 hr) at 295 K | -- |
F | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The octahedral crystals were rapid growth (approximately 24 hr) at 295 K | -- |
G | 8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 6.8. | The Octahedral crystals were rapid growth (approximately 24 hr) at 295 K | >5.0 Å |
H | 14 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.3 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 8.0. | The splintered off and crowded together crystals were rapid growth (approximately 24 hr) at 295 K | -- |
I | 22 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.3 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The hexahedral octahedral crystals were Slow growth (approximately 7 d) at 295 K | -- |
J | 14 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.25 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The typical octahedral crystals were Slow growth (approximately 1-2 d) at 295 K | 2.9–3.0 Å |
K | 22 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The dodecahedral crystals were slow growth (approximately 24 hr) at 295 K | -- |
L | 22 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The dodecahedral crystals were Slow growth (approximately 9d) at 295 K | -- |