Table 3 The crystallization solutions of ammonium sulfate
From: The development and application of new crystallization method for tobacco mosaic virus coat protein
Sample | Protein concentration and buffera | Crystallization bufferb | Temperature (K) |
|---|---|---|---|
WT-TMV-CP32 (cleaved GST) | 8 –14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 | 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5 | 277/295 |
WT-His-TMV-CP12 | 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 | 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5 | 277/295 |
TR-His-TMV-CP19 | 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 | 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5 | 277/295 |
TR-His-TMV-CP68 | 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 | 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5 | 277/295 |
TR-His-TMV-CP62 | 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 | 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5 | 277/295 |