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Table 1 DNA sequences of the primers

From: The development and application of new crystallization method for tobacco mosaic virus coat protein

serial number

Name

Primers

PrimercDNA

TMV-cDNA

5’-TCGACATAGGGACATCTTC-3’

Primer1a,c

WT-TMV-CP-FOR

5’-GGAATTCCATATG TCTTACAGTATCACTACTCC-3’

Primer2b,d

WT-TMV-CP-REV

5’- CCGCTCGAG TCAAGTTGCAGGACCAGAGG-3’

Primer3b

TR-TMV-CP-FOR

5’-CGGGATCC ATGTCTTACAGTATCACTACTCC-3’

Primer6a,d,e

TR-His-TMV-CP-FOR

5’-GGAATTCCATATG ATCACTACTCCATCACAGTTCG-3’

Primer8e

TR-His-TMV-CP-REV

5’-CCGCTCGAG TCAACCAGAGGTCCAAACCAA-3’

Primer9c

TR-His-TMV-CP-REV

5’-CCGCTCGAG TCAAGTTGCAGGACCAGAGG-3’

  1. aThe WT-His-TMV-CP12 fragment was amplified by using primer1 and primer2.
  2. bThe WT-GST-TMV-CP32 fragment was amplified by using primer3 and primer2.
  3. cThe TR-His-TMV-CP19 fragment was amplified by using primer1and primer9.
  4. dThe TR-His-TMV-CP62 fragment was amplified by using primer6 and primer2.
  5. eThe TR-His-TMV-CP68 fragment was amplified by using primer6 and primer8.