Figure 3
TASK channel function is not required for suppression of HIV-1 transcription. A. 293T cells were transfected with pNL4-3Luc and either wild type TASK-1 or non-functional TASK protein expression vectors as described in the text. After 48 hours a luciferase assay was performed to determine the effect of ion channel function on TASK-1’s ability to suppress HIV-1 transcription. Data shown are normalized to empty vector control (EV). B. 293T cells were transfected with pNL4-3Luc and wild type TASK-1 in the presence of the ion channel inhibitor 5mM BaCl2. After 48 hours a luciferase assay was performed to measure HIV-1 transcription. Data are normalized to the empty vector control (EV). C. Transfected 293T cells were harvested, lysed and equal amounts of cell lysate protein were subjected to 4-12% SDS-PAGE. After transfer to nitrocellulose membranes, TASK and β-tubulin proteins were revealed by immunoblotting with polyclonal antibodies followed by chemilumenescence detection. (* P < .001; Statistical analysis was performed on the samples using a student T test).