Figure 1

TASK-1 suppresses HIV-1 replication. A. 293T cells were co-transfected with pNL4-3 and TASK-1 expression vector or empty vector. After 48 hours supernatants were collected and a p24 ELISA was performed. Data are normalized to the empty vector control (EV). B. TASK-1 expression in transfected cells. 293T cells were harvested and lysed with buffer containing protease inhibitors. Equal amounts of protein from cell lysates were separated by 4-12% SDS-PAGE and transferred onto nitrocellulose membranes. TASK and β-tubulin proteins were detected by immunoblotting using polyclonal antibodies and visualization by chemilumenescence. C. TASK-1 does not decrease HIV-1 Gag virus-like particle release. 293T cells were co-transfected with plasmid encoding Gag driven by CMV promoter and TASK-1 or empty vector. After 48 hours supernatants were collected, and a p24 ELISA was performed. Data are normalized to the empty vector control (EV). D. TASK-1 expression in transfected cells from (C). 293T cells from (C.) were harvested and lysed with a detergent buffer containing protease inhibitors. Equal protein amounts of cell lysate were separated by 4-12% SDS-PAGE and transferred to nitrocellulose membranes. TASK and β-tubulin proteins were revealed by immunoblotting using polyclonal antibodies followed by chemilumenescence detection. (* P < .0001; Student T test was used to determine statistical significance).