Figure 4

(A, B, C, D) Effects of siRNAs on IFN-α, IFN-β, TNF-α in HEK293 and HepG2.2.15 cells. (A) HEK293 and HepG2.2.15 cells were respectively transfected with pU6, S1, S2, S3, siHsc70, EGFP-siRNA and the positive-control poly (I:C). The concentrations of IFN-α, IFN-β and TNF-α in cell culture supernatants from transfected cells were determined by ELISA assay. (B) The effects of siRNAs on the mRNA concentrations of IFN-α, IFN-β, and TNF-α in HEK293 cells. HEK293 and HepG2.2.15 cells were respectively transfected with pU6, S1, S2, S3, siHsc70, EGFP-siRNA and the positive-control poly (I:C). The mRNA concentrations of IFN-α, IFN-β and TNF-α were examined by RT-PCR amplification with the SYBR RT-PCR Kit. Data represent means±SD from three independent experiments were performed in triplicate. (C) T98G cells were transfected with pU6, S1, S2, S3, siHsc70, EGFP-siRNA and the positive-control poly (I:C). The concentrations of IFN-α, IFN-β and TNF-α in cell culture supernatants from transfected cells were determined by ELISA assay. (D) The effects of siRNAs on the mRNA concentrations of IFN-α, IFN-β, and TNF-α in HEK293 cells. HEK293 cells were transfected with pU6, S1, S2, S3, siHsc70, EGFP-siRNA and the positive-control poly (I:C). The mRNA concentrations of IFN-α, IFN-β and TNF-α were examined by RT-PCR amplification with the SYBR RT-PCR Kit. Data represent means±SD from three independent experiments were performed in triplicate.