Figure 2
From: Anti-HBV efficacy of combined siRNAs targeting viral gene and heat shock cognate 70
Synergistic inhibition of HBV protein expression by siRNA targeting viral gene in combination with siHsc70 in HepG2.2.15 cells. (A) The expression of Hsc70 in HepG2.2.15 cells transfected with siHsc70 or siCtrl after 72 hrs was identified by Western blotting with anti-Hsc70. GAPDH expression was used as an internal control. (B) The reduction of HBsAg expression. HepG2.2.15 cells transfected with pU6, S1, S2, siHsc70, S2 with siHsc70, and also the heterologous S3. (C) The reduction of HBeAg expression. HepG2.2.15 cells transfected with pU6, S1, S2, siHsc70, S2 with siHsc70, and also the heterologous S3. Data represent means±SD from three independent experiments were performed in triplicate.*Significant differences compared to the heterologous siRNA control: Student’s t-test; P < 0.05.