Figure 1From: Anti-HBV efficacy of combined siRNAs targeting viral gene and heat shock cognate 70Effect of siRNAs on the expression of HBV surface open reading frame in HEK293 and T98G cells. (A) As shown in Additional file 2: Figure S2. (B) Fluorescence micrographs of cells transfected with reporter plasmids and cotransfected with either the corresponding or non-corresponding siRNA with Lipofectamine TM 2000 (Invitrogen). At 24 hrs after transfection, the cells were observed with an Olympus BH-2 microscope, and representative bright-field images (left column) and relative fluorescent-field images (right column) were recorded by tenfold amplification. (C) Flow cytometry analysis of siRNA-mediated gene silencing of EGFP. Level of EGFP expression in cells cotransfected with (a) pEGFP-N1 vector; ( b) pS-EGFP-N1 and pU6; (c) pS-EGFP-N1 and S1; (d) pS-EGFP-N1 and S2; (e) pS-EGFP-N1 and S3 (heterologous siRNA). (D) Cells were analyzed for EGFP expression by fluorescence-activated cell sorting and the level of fluorescence relative to the control was quantitated. The mean fluorescence intensity of control siRNA was taken as 100% and adopted as control. Data represent means±SD from three independent experiments were performed in triplicate.*Significant differences compared to the heterologous siRNA control: Student’s t-test; P < 0.05.Back to article page