Figure 3

Sustained Tat-SF1 suppression inhibits HIV-1 replication in CD4+ T cell-derived SupT1 cells. SupT1 cell lines, with stable shRNA expression generated by lentiviral transduction, were infected with HIV-1^p81A-4 at a TCID₅₀ of 50/ml, in triplicate. 3A. Levels of p24 in culture supernatant were determined by ELISA 0, 2, 4, 7, 10, 14 and 17 days post-infection. Crosses indicate discontinued p24 measurement as a result of cell death. 3B. p24 levels in SupT1 cells expressing either shLTR-U5 or shhtatsf1-a, relative to the U6 mock, at days 10 and 14 post-infection. Data are expressed as the mean ± SEM.