Figure 2

Tat-SF1 suppression inhibits HIV-1 infectious particle production from TZM-bl cells. 2A. Schematic of the HIV-1 infection protocol. 2B. TZM-bl cell lysates were analysed for luciferase activity 72 h post-transfection and 48 h post-infection with HIV-1^p81A-4 at a TCID₅₀ of 1000/ml, in triplicate. shLTR-U5 and shTAT, which target viral RNAs, were included as positive controls. 2C. Untransfected TZM-bl cell lysates were analysed for luciferase activity 48 h post-incubation with culture supernatant isolated from shRNA-expressing TZM-bl cells. Luciferase activity is given relative to culture supernatant p24 concentration, determined by ELISA. Data are expressed as the mean ± SEM. *, p <0.05, one-way ANOVA with Dunnett post-tests relative to mock construct, U6.