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Figure 2 | Virology Journal

Figure 2

From: Development of a SYBR green I based RT-PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti

Figure 2

Comparative sensitivity of SYBR Green I real-time RT-PCR assay vs. conventional RT-PCR. (A) Sensitivity of YFV RT-PCR assay as shown in the amplification plot (curves from left to right corresponds to decreasing concentration of YFV from 105 to 1 PFU/mL). The detection limit for the assay was 1 PFU/mL. (B) Sensitivity of conventional RT-PCR as observed by 113 bp amplicon on 2% agarose gel with a detection limit of 10 PFU/mL. Lane M: 100 bp DNA ladder (Fermentas, USA); lanes from 1 to 6 corresponds to decreasing concentration of YFV from 105 to 1 PFU/mL.

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Virology Journal

ISSN: 1743-422X