Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

Table 1 PRRS virus isolation efficiency from swine samples using SJPL cells compared to MARC-145 cells

Amount of PRRS virus in tissues^a	Cell types
Amount of PRRS virus in tissues^a	MARC-145	SJPL
(TCID₅₀/g)	(number isolated/number tested)^b	(number isolated/number tested)
0-100^c	0 / 7	0 / 7
101-500	2 / 6	0 / 6
501-2500	7 / 7	6 / 7
2501-40000	2 / 2	2 / 2
Total:	11 / 22	8 / 22

^aThe PRRSV was quantified as previously described using a real-time PCR assay [67].
^bThe virus isolation were attempted with both cell lines using the same swine tissue homogenates.
^cThree cases out of 7 were real-time PCR negative for the presence of PRRSV.

ISSN: 1743-422X