From: Evaluation of nanoparticle tracking analysis for total virus particle determination
EPD | NTA | ||||||
---|---|---|---|---|---|---|---|
Fraction | Volume (ml) | PFU/ml | PFU total | PFU rec.(%) | VP/ml | VP total | VP rec. (%) |
Load | 36 | 1,00E+08 | 3,60E+09 | 1,03E+10 | 3,71E+11 | ||
Flow through 1 | 10,0 | 6,31E+02 | 6,31E+03 | 0,0 | 1,12E+08 | 1,12E+09 | 0,3 |
Flow through 2 | 26,0 | 1,00E+03 | 2,60E+04 | 0,0 | 1,59E+08 | 4,13E+09 | 1,1 |
Wash | 23,0 | NA | 3,03E+08 | 6,97E+09 | 2 | ||
Elution 1 | 1,2 | 2,00E+09 | 2,40E+09 | 66,7 | 1,79E+11 | 2,14E+11 | 57,8 |
Elution 2 | 3,0 | 6,31E+06 | 1,89E+07 | 0,5 | 1,14E+09 | 3,41E+09 | 1 |
SUM | 67,2 | 62,0 |