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Table 4 Comparison of EPD and NTA method

From: Evaluation of nanoparticle tracking analysis for total virus particle determination

  

EPD

 

NTA

 

Fraction

Volume (ml)

PFU/ml

PFU total

PFU rec.(%)

VP/ml

VP total

VP rec. (%)

Load

36

1,00E+08

3,60E+09

 

1,03E+10

3,71E+11

 

Flow through 1

10,0

6,31E+02

6,31E+03

0,0

1,12E+08

1,12E+09

0,3

Flow through 2

26,0

1,00E+03

2,60E+04

0,0

1,59E+08

4,13E+09

1,1

Wash

23,0

NA

  

3,03E+08

6,97E+09

2

Elution 1

1,2

2,00E+09

2,40E+09

66,7

1,79E+11

2,14E+11

57,8

Elution 2

3,0

6,31E+06

1,89E+07

0,5

1,14E+09

3,41E+09

1

SUM

   

67,2

  

62,0

  1. Analysis of adenovirus purification on CIM QA monolithic column by NTA and EPD assay.