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Table 3 Accuracy of NTA determined by spike-and-recovery method

From: Evaluation of nanoparticle tracking analysis for total virus particle determination

  Adenovirus harvest CIM QA purified adenovirus
Dilution of Ad sample for NTA analysis 30 500
Theoretical latex spike (LP/5μl) 5,25E+08 5,25E+08
Measured latex spike (LP/5μl) 5,15E+08 5,42E+08
Concentration of non-spiked Ad sample (VP/ml) 3,43E+08 3,57E+08
Concentration of spiked Ad sample (particles/ml) 7,84E+08 8,27E+08
∆ (spiked Ad sample-non-spiked Ad sample) 4,42E+08 4,70E+08
Recovery of latex spike sample according to measured spike (%) 85,7 86,7
Recovery of latex spike sample according to theoretical spike (%) 84,1 89,5