Table 3 Accuracy of NTA determined by spike-and-recovery method
From: Evaluation of nanoparticle tracking analysis for total virus particle determination
Adenovirus harvest | CIM QA purified adenovirus | |
|---|---|---|
Dilution of Ad sample for NTA analysis | 30 | 500 |
Theoretical latex spike (LP/5μl) | 5,25E+08 | 5,25E+08 |
Measured latex spike (LP/5μl) | 5,15E+08 | 5,42E+08 |
Concentration of non-spiked Ad sample (VP/ml) | 3,43E+08 | 3,57E+08 |
Concentration of spiked Ad sample (particles/ml) | 7,84E+08 | 8,27E+08 |
∆ (spiked Ad sample-non-spiked Ad sample) | 4,42E+08 | 4,70E+08 |
Recovery of latex spike sample according to measured spike (%) | 85,7 | 86,7 |
Recovery of latex spike sample according to theoretical spike (%) | 84,1 | 89,5 |