Dynamically tiled layout of the amplicons across the targeted genome. This is an example of the tiling output from a single primer design run performed on RUBV-G1. Each green rectangle represents an amplicon. The long black bar represents the targeted region and the red X’s, above the targeted region, represent locations where 2x coverage could not be achieved. Amplicon lengths for RUBV-G1 and RUBV-G2 using the high GC protocol were targeted to be between 300–350 bp.