Figure 4

Neutralizing activity of pooled sera. (A) Sera from animals immunized with p15E (white columns), gp70 (gray columns), p15E and gp70 (dark gray columns) and control animals (black columns) were pooled and analyzed. The delta ct mean of three experiments and the corresponding standard deviation are shown. (B) Shown are the results of a second experiment and the Student´s T-test evaluation of both experiments. (C) Increase in the neutralization titer analyzing the pooled sera from 4 animals immunized with gp70 and p15E. 1 – preimmune serum, 2 –first bleeding 22 days after the first immunization, 3 –bleeding after the second immunization, 4 –bleeding after the third immunization. 4 – bleeding after the fourth immunization.