Figure 3

Epitope mapping of the immune sera using two different methods. (A) Example of an epitope mapping using membranes: serum from hamster 1/3, immunized with p15E. 15-mer peptides overlapping by 12 residues were fixed on the membrane, stained peptides were identified and epitopes determined. (B) Example of an epitope mapping using glass slides and the same peptides as in A. Hamster 3/4 was immunized with p15E and gp70 together. (C) Summary of the epitopes recognized by two immune sera from animals immunized with p15E (1/1, 1/3) and two sera from animals immunized with p15E and gp70 (3/3, 3/4). The most common epitopes recognized after immunization of goats, rats, mice, and guinea pigs with p15E [4, 12] were framed. The peptides E1 and E2 used for screening of the sera were indicated.