Skip to main content

Table 1 Candidate epitopes and their affinity for HLA-A*0201 molecule of T2 cells

From: HLA-A*0201-restricted CD8+T-cell epitopes identified in dengue viruses

Peptides

Serotypes

Positiona

Sequencesb

Scorec

FId

ELISPOT SFCs/1×105cells

ICS (%)e CD8+IFN-γ+T cells

    

SYFPEITHI

1 μg

10 μg

100 μg

  

D1V-NS4a56-64

D1V

NS4a (56-64)

MLLALIAVL

29

0.05

0.05

0.08

  

D1V-C46-54

D1V

C (46-54)

LVMAFMAFL

20

0.01

0.02

0.02

  

D1V-NS4b562-570

D1V

NS4b (562-570)

LLATSIFKL

30

0.01

0.05

0.05

  

D1V-NS2a169-177

D1V

NS2a (169-177)

AMVLSIVSL

28

0.03

0.05

0.04

  

D1V-NS4a140-148

D1V

NS4a (140-148)

GLLFMILTV

30

0.28

0.85

4.5

58 ± 8

0.71 ± 0.11

D2V-NS4a140-148

D2V

NS4a (140-148)

AILTVVAAT

23

0.1

0.2

1.2

10 ± 3

0.12 ± 0.05

D3V-NS4a140-148

D3V

NS4a (140-148)

GILTLAAIV

24

0.1

0.15

4.05

37 ± 6

0.37 ± 0.08

D4V-NS4a140-148

D4V

NS4a (140-148)

TILTIIGLI

22

0.15

0.3

1.08

17 ± 5

0.21 ± 0.05

D1V-NS2a144-152

D1V

NS2a (144-152)

QLWAALLSL

28

0.05

0.22

1.12

36 ± 6

0.41 ± 0.07

D2V-NS2a144-152

D2V

NS2a (144-152)

QLAVTIMAI

25

0.24

0.39

3.85

21 ± 3

0.21 ± 0.05

D3V-NS2a144-152

D3V

NS2a (144-152)

QLWTALVSL

29

0.2

0.45

2.6

62 ± 9

0.79 ± 0.15

D4V-NS2a143-151

D4V

NS2a (143-151)

QVGTLALSL

18

0.22

0.2

0.8

12 ± 3

0.13 ± 0.06

D1V-NS4b183-191

D1V

NS4b (183-191)

LLMRTTWAL

26

0.29

0.4

1.95

42 ± 7

0.44 ± 0.09

D2V-NS4b182-190

D2V

NS4b (182-190)

LMMRTTWAL

24

0.58

3.42

4.8

37 ± 5

0.37 ± 0.09

D3V-NS4b182-190

D3V

NS4b (182-190)

LLMRTSWAL

24

0.22

0.65

3.6

24 ± 5

0.27 ± 0.07

D4V-NS4b179-187

D4V

NS4b (179-187)

LLMRTTWAF

16

0.2

0.59

4.4

22 ± 4

0.16 ± 0.06

  1. a Position was determined according to the indicated DV serotype.
  2. b Altered peptides representing heterologous DV serotypes. Bolded letters indicate the variant amino acid.
  3. c Score predicted by online prediction algorithms SYFPEITHI.
  4. d FI = [Mean fluorescence intensity(MFI) sample-MFI background]/MFI background.
  5. e The proportion of CD8+IFN-γ+T cells in CD8+Tcells.