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Table 1 Candidate epitopes and their affinity for HLA-A*0201 molecule of T2 cells

From: HLA-A*0201-restricted CD8+T-cell epitopes identified in dengue viruses

Peptides Serotypes Positiona Sequencesb Scorec FId ELISPOT SFCs/1×105cells ICS (%)e CD8+IFN-γ+T cells
     SYFPEITHI 1 μg 10 μg 100 μg   
D1V-NS4a56-64 D1V NS4a (56-64) MLLALIAVL 29 0.05 0.05 0.08   
D1V-C46-54 D1V C (46-54) LVMAFMAFL 20 0.01 0.02 0.02   
D1V-NS4b562-570 D1V NS4b (562-570) LLATSIFKL 30 0.01 0.05 0.05   
D1V-NS2a169-177 D1V NS2a (169-177) AMVLSIVSL 28 0.03 0.05 0.04   
D1V-NS4a140-148 D1V NS4a (140-148) GLLFMILTV 30 0.28 0.85 4.5 58 ± 8 0.71 ± 0.11
D2V-NS4a140-148 D2V NS4a (140-148) AILTVVAAT 23 0.1 0.2 1.2 10 ± 3 0.12 ± 0.05
D3V-NS4a140-148 D3V NS4a (140-148) GILTLAAIV 24 0.1 0.15 4.05 37 ± 6 0.37 ± 0.08
D4V-NS4a140-148 D4V NS4a (140-148) TILTIIGLI 22 0.15 0.3 1.08 17 ± 5 0.21 ± 0.05
D1V-NS2a144-152 D1V NS2a (144-152) QLWAALLSL 28 0.05 0.22 1.12 36 ± 6 0.41 ± 0.07
D2V-NS2a144-152 D2V NS2a (144-152) QLAVTIMAI 25 0.24 0.39 3.85 21 ± 3 0.21 ± 0.05
D3V-NS2a144-152 D3V NS2a (144-152) QLWTALVSL 29 0.2 0.45 2.6 62 ± 9 0.79 ± 0.15
D4V-NS2a143-151 D4V NS2a (143-151) QVGTLALSL 18 0.22 0.2 0.8 12 ± 3 0.13 ± 0.06
D1V-NS4b183-191 D1V NS4b (183-191) LLMRTTWAL 26 0.29 0.4 1.95 42 ± 7 0.44 ± 0.09
D2V-NS4b182-190 D2V NS4b (182-190) LMMRTTWAL 24 0.58 3.42 4.8 37 ± 5 0.37 ± 0.09
D3V-NS4b182-190 D3V NS4b (182-190) LLMRTSWAL 24 0.22 0.65 3.6 24 ± 5 0.27 ± 0.07
D4V-NS4b179-187 D4V NS4b (179-187) LLMRTTWAF 16 0.2 0.59 4.4 22 ± 4 0.16 ± 0.06
  1. a Position was determined according to the indicated DV serotype.
  2. b Altered peptides representing heterologous DV serotypes. Bolded letters indicate the variant amino acid.
  3. c Score predicted by online prediction algorithms SYFPEITHI.
  4. d FI = [Mean fluorescence intensity(MFI) sample-MFI background]/MFI background.
  5. e The proportion of CD8+IFN-γ+T cells in CD8+Tcells.