|
Primer sequence (5′ – 3′)
|
Annealing Temp/Cycles
|
Nucleotides amplified*
|
Amplicon size
|
---|
E6 F
|
AGTAACCGAAAACGGTCGGGA
|
55°C/40 cycles
|
38-491
|
454 pb
|
E6 R
|
GTTGTGAAATCGTCGTTTTTCA
| | | |
E7 F
|
TGAAAAACGACGATTTCACAAC
|
55°C/40 cycles
|
470-931
|
462 pb
|
E7 R
|
ACCTTCTGGATCAGCCATTG
| | | |
E4 F
|
GTAAAGGAAGGGTACAACACG
|
57°C/35 cycles
|
3309-3792
|
484 pb
|
E4 R
|
CTGTCCAATGCCAGGTGGA
| | | |
LCR 1 F
|
TCGGTTGCCTTTGGCTTAT
|
55°C/40 cycles
|
7465-7775
|
311 pb
|
LCR 1 R
|
AAGGGTAGACAGAATGTTGGACA
|
55°C/40 cycles
|
7718-163
|
303 pb
|
LCR 2 F
|
GCTAATTGCATACTTGGCTTG
| | | |
LCR 2 R
|
TCCGTGCACAGATCAGGTAG
| | | |
MY11
|
GCACAGGGTCATAACAATGG
|
55°C/40 cycles
|
6558-7012
|
455 pb
|
MY09
|
CGTCCAAGGGATATTGATC
| | | |
L1 seq
|
ACAGTCTCCTGTACCTGGG
| | | |
- *Position numbering refers to the first nucleotide of the HPV 18 reference genome (accession number NC001357). F: forward primer, R: reverse primer.