Table 3 Polymerase chain reaction characteristics for Human papillomavirus 18
Primer sequence (5′ – 3′) | Annealing Temp/Cycles | Nucleotides amplified* | Amplicon size | |
|---|---|---|---|---|
E6 F | AGTAACCGAAAACGGTCGGGA | 55°C/40 cycles | 38-491 | 454 pb |
E6 R | GTTGTGAAATCGTCGTTTTTCA | |||
E7 F | TGAAAAACGACGATTTCACAAC | 55°C/40 cycles | 470-931 | 462 pb |
E7 R | ACCTTCTGGATCAGCCATTG | |||
E4 F | GTAAAGGAAGGGTACAACACG | 57°C/35 cycles | 3309-3792 | 484 pb |
E4 R | CTGTCCAATGCCAGGTGGA | |||
LCR 1 F | TCGGTTGCCTTTGGCTTAT | 55°C/40 cycles | 7465-7775 | 311 pb |
LCR 1 R | AAGGGTAGACAGAATGTTGGACA | 55°C/40 cycles | 7718-163 | 303 pb |
LCR 2 F | GCTAATTGCATACTTGGCTTG | |||
LCR 2 R | TCCGTGCACAGATCAGGTAG | |||
MY11 | GCACAGGGTCATAACAATGG | 55°C/40 cycles | 6558-7012 | 455 pb |
MY09 | CGTCCAAGGGATATTGATC | |||
L1 seq | ACAGTCTCCTGTACCTGGG |